- 表达克隆[通过进行基因表达而克隆目的基因的方法
- 表达克隆化
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expression cloning中文什么意思
例句与用法
- In our study we have cloned the osd gene from s . typhimurium by pcr , characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd
再将hpylori尿素酶b亚单位基因与尿素酶b和热休克蛋白a融合基因分别克隆入ptrc99a一asd质粒的多克隆位点之内。 - Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible
结论成功构建了echistatin的原核高效表达克隆，表达量高于现有国内外研究水平，为echistatin功能及相关疾病的研究奠定了基础。 - In the first part , the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative , to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above
方法： （ ）以融合蛋白prpap通过瞬时表达克隆法筛选两个cdna文库，或者通过与胚胎脑的结合实验筛选有丰富结合蛋白的脑区，以图构建cdna文库并进行表达克隆的筛选。 - Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220 ，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。
百科释义
Expression cloning is a technique in DNA cloning that uses expression vectors to generate a library of clones, with each clone expressing one protein. This expression library is then screened for the property of interest and clones of interest recovered for further analysis.
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